maxima h minus reverse transcriptase (200u/μl) with 5×rt buffer (Thermo Fisher)
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maxima h minus reverse transcriptase (200u/μl) with 5×rt buffer
Maxima H Minus Reverse Transcriptase (200u/μl) With 5×Rt Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5%C3%97+reverse+transcriptase+(rt)+buffer/maxima+h+minus+reverse+transcriptase/pmc11539149-15-7-10
Average 90 stars, based on 1 article reviews
Maxima H Minus Reverse Transcriptase (200u/μl) With 5×Rt Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5%C3%97+reverse+transcriptase+(rt)+buffer/maxima+h+minus+reverse+transcriptase/pmc11539149-15-7-10
Average 90 stars, based on 1 article reviews
maxima h minus reverse transcriptase (200u/μl) with 5×rt buffer - by Bioz Stars,
2026-09
90/100 stars
Images
1) Product Images from "Time course transcriptomic profiling suggests Crp/Fnr transcriptional regulation of nosZ gene in a N 2 O-reducing thermophile"
Article Title: Time course transcriptomic profiling suggests Crp/Fnr transcriptional regulation of nosZ gene in a N 2 O-reducing thermophile
Journal: iScience
doi: 10.1016/j.isci.2024.111074
Figure Legend Snippet:
Techniques Used: Virus, Recombinant, Reverse Transcription, Isolation, Control, Sequencing, Mass Spectrometry, Software, In Silico, Targeted Proteomics
Related Articles
other:Article Title: A double-Flp-in method for stable overexpression of two genes Article Snippet: After this, 6 μL 5 × RT buffer ( Article Title: Polynucleotides and polypeptides involved in plant fiber development and methods of using same Article Snippet: Reverse transcription was effected using 1.5 μg total RNA, using 300 U Article Title: Inverse Impact of Cancer Drugs on Circular and Linear RNAs in Breast Cancer Cell Lines Article Snippet: Purified RNA was quantified with NanoDropTM 1000 Spectrophotometer (Thermo Fisher Scientific, Invitrogen, Monza, Italy) and 2 μg reverse transcribed using Article Title: RAG-seq: NSR-primed and Transposase Tagmentation-mediated Strand-specific Total RNA Sequencing in Single Cells Article Snippet: Next, 5 μl RT mixture [2 μl 5× RT buffer, 100 U Maxima H Minus Reverse Transcriptase (Catalog No. EP0751, Article Title: Short and Long-Read Sequencing Survey of the Dynamic Transcriptomes of African Swine Fever Virus and the Host Cells Article Snippet: After 5 min of incubation at 65°C, the following components were added: 5 × Extraction:Article Title: Osteogenesis and osteoclastogenesis on a chip: Engineering a self-assembling 3D coculture. Article Snippet: RNA was isolated using the Qiagen RNeasy kit (Qiagen, Hilden, Germany) following supplier instructions including a 15 min DNAse incubation step (Qiagen; 74106) to remove genomic DNA contamination. .. After extraction, RNA quantity and purity were assessed with a spectrophotometer (NanoDropTM One, Isogen Life Science, The Netherlands). cDNA was synthesized in a thermal cycler (protocol: 65 ◦C (5 min), on ice (2 min) while adding the enzyme mixture, 37 ◦C (2 min), 25 ◦C (10 min), 37 ◦C (50 min), and 70 ◦C (15 min)) starting from a 20 μL reaction solution containing 200 ng of RNA, 1 μL dNTPs (10 mM, Invitrogen), 1 μL random primers (50 ng/μL, Promega, C1181), 2 μL 0.1 M DTT, 4 μL 5× first strand buffer, 1 μL Spectrophotometry:Article Title: Osteogenesis and osteoclastogenesis on a chip: Engineering a self-assembling 3D coculture. Article Snippet: RNA was isolated using the Qiagen RNeasy kit (Qiagen, Hilden, Germany) following supplier instructions including a 15 min DNAse incubation step (Qiagen; 74106) to remove genomic DNA contamination. .. After extraction, RNA quantity and purity were assessed with a spectrophotometer (NanoDropTM One, Isogen Life Science, The Netherlands). cDNA was synthesized in a thermal cycler (protocol: 65 ◦C (5 min), on ice (2 min) while adding the enzyme mixture, 37 ◦C (2 min), 25 ◦C (10 min), 37 ◦C (50 min), and 70 ◦C (15 min)) starting from a 20 μL reaction solution containing 200 ng of RNA, 1 μL dNTPs (10 mM, Invitrogen), 1 μL random primers (50 ng/μL, Promega, C1181), 2 μL 0.1 M DTT, 4 μL 5× first strand buffer, 1 μL Synthesized:Article Title: Osteogenesis and osteoclastogenesis on a chip: Engineering a self-assembling 3D coculture. Article Snippet: RNA was isolated using the Qiagen RNeasy kit (Qiagen, Hilden, Germany) following supplier instructions including a 15 min DNAse incubation step (Qiagen; 74106) to remove genomic DNA contamination. .. After extraction, RNA quantity and purity were assessed with a spectrophotometer (NanoDropTM One, Isogen Life Science, The Netherlands). cDNA was synthesized in a thermal cycler (protocol: 65 ◦C (5 min), on ice (2 min) while adding the enzyme mixture, 37 ◦C (2 min), 25 ◦C (10 min), 37 ◦C (50 min), and 70 ◦C (15 min)) starting from a 20 μL reaction solution containing 200 ng of RNA, 1 μL dNTPs (10 mM, Invitrogen), 1 μL random primers (50 ng/μL, Promega, C1181), 2 μL 0.1 M DTT, 4 μL 5× first strand buffer, 1 μL Reverse Transcription:Article Title: Osteogenesis and osteoclastogenesis on a chip: Engineering a self-assembling 3D coculture. Article Snippet: RNA was isolated using the Qiagen RNeasy kit (Qiagen, Hilden, Germany) following supplier instructions including a 15 min DNAse incubation step (Qiagen; 74106) to remove genomic DNA contamination. .. After extraction, RNA quantity and purity were assessed with a spectrophotometer (NanoDropTM One, Isogen Life Science, The Netherlands). cDNA was synthesized in a thermal cycler (protocol: 65 ◦C (5 min), on ice (2 min) while adding the enzyme mixture, 37 ◦C (2 min), 25 ◦C (10 min), 37 ◦C (50 min), and 70 ◦C (15 min)) starting from a 20 μL reaction solution containing 200 ng of RNA, 1 μL dNTPs (10 mM, Invitrogen), 1 μL random primers (50 ng/μL, Promega, C1181), 2 μL 0.1 M DTT, 4 μL 5× first strand buffer, 1 μL Article Title: Dissociation and inDrops microfluidic encapsulation of human gut tissues for single-cell atlasing studies Article Snippet: Maxima H Minus Reverse transcriptase , Thermo Scientific , EP0753. .. Article Title: Ribosome-dependent conformational flexibility changes and RNA dynamics of IRES domains revealed by differential SHAPE. Article Snippet: .. Treated and untreated RNA (2 pmol) was incubated with the fluorescent primer 5′-NED-TAGCCTTATGCAGTTGCTCTCC (0.1 μM) at 65 °C for 5 min, then at 35 °C 5 min, and 4 °C 1 min. Primer extension reactions were conducted in a final volume of 16 μl containing reverse Incubation:Article Title: Ribosome-dependent conformational flexibility changes and RNA dynamics of IRES domains revealed by differential SHAPE. Article Snippet: .. Treated and untreated RNA (2 pmol) was incubated with the fluorescent primer 5′-NED-TAGCCTTATGCAGTTGCTCTCC (0.1 μM) at 65 °C for 5 min, then at 35 °C 5 min, and 4 °C 1 min. Primer extension reactions were conducted in a final volume of 16 μl containing reverse |